王娜，女，1980年3月生人，于2008年3月獲中國科學(xué)院水生生物研究所遺傳學(xué)博士學(xué)位，現(xiàn)任中國水產(chǎn)科學(xué)研究院黃海水產(chǎn)研究所研究員，碩士生導(dǎo)師。主要從事魚類細(xì)胞種質(zhì)資源保存應(yīng)用及功能基因組學(xué)研究，主持國家自然科學(xué)基金面上項(xiàng)目2項(xiàng)、青年基金項(xiàng)目1項(xiàng)，國家863子課題1項(xiàng)，十三五藍(lán)色糧倉子課題1項(xiàng)，十四五重點(diǎn)研發(fā)計(jì)劃課題1項(xiàng)等，發(fā)表論文50多篇，其中第一或通訊作者SCI文章28篇、核心期刊2篇，第一發(fā)明人獲授權(quán)發(fā)明專利4項(xiàng)，參編專著2部。作為主要完成人獲得省部級獎(jiǎng)勵(lì)12項(xiàng)。獲山東省泰山學(xué)者青年專家稱號。
聯(lián)系電話：15854263035
通訊地址：山東省青島市南京路106號中國水產(chǎn)科學(xué)研究院黃海水產(chǎn)研究所
郵政編碼：266071
E-mail：wangna@www.energicube.com
主要研究內(nèi)容、研究方向
1. 半滑舌鰨等魚類細(xì)胞種質(zhì)資源保存應(yīng)用技術(shù)研究：開發(fā)研究適宜于半滑舌鰨等魚類細(xì)胞系的高效基因?qū)爰夹g(shù)體系，為魚類功能基因組學(xué)研究提供高效平臺；優(yōu)化半滑舌鰨等魚類的魚類生殖/胚胎干細(xì)胞建立及干性特征分析，為魚類優(yōu)異品系培育及瀕危魚類保護(hù)提供重要細(xì)胞資源。
2. 半滑舌鰨等魚類性別和生長關(guān)聯(lián)機(jī)制研究：運(yùn)用組學(xué)測序等方法開展半滑舌鰨等性別生長異形魚類在不同性別個(gè)體生長-生殖軸的多組學(xué)機(jī)制分析；利用分子生物學(xué)實(shí)驗(yàn)手段開展關(guān)鍵基因的表觀、轉(zhuǎn)錄及蛋白互作機(jī)制研究；采用RNA 干擾、基因組編輯等方法揭示關(guān)鍵基因在細(xì)胞系、組織或成體水平的作用機(jī)制。
3. 半滑舌鰨等魚類性別生長異形關(guān)鍵基因在基因組編輯育種中的應(yīng)用潛能研究：借助生物兼容性納米材料、電穿孔技術(shù)等，優(yōu)化建立半滑舌鰨等魚類高效CRISPR-Cas9 基因組編輯技術(shù)；開展性別生長異形關(guān)鍵基因的基因組編輯敲除或敲入的探索研究，加快高產(chǎn)優(yōu)質(zhì)魚類品系的精準(zhǔn)培育進(jìn)程。
代表性論著（10篇以內(nèi)）
1. Shi R#, Li XH#, Xu XW, Chen ZF, Zhu Y*, Wang N*. Genome-wide analysis of BMP/GDF family and DAP-seq of YY1 suggest their roles in Cynoglossus semilaevis sexual size dimorphism. International Journal of Biological Macromolecules, 2023, 253 (5): 127201.
2. Gong Z#, Zhang Q#, Liu J, Hu G, Chen S*, Wang N*. Establishment, characterization and application in germplasm conservation and disease resistance: An embryonic cell line from Yangtze sturgeon (Acipenser dabryanus). Aquaculture, 2023, 575:739807.
3. Li X, Yang Q, Shi R, Xu X, Chen Z, Chen S, Wang N*. Genome-wide identification of insulin-like growth factor-binding protein family in Chinese tongue sole (Cynoglossus semilaevis) and their responses to sex steroids. Aquaculture 2022; 557: 738346
4. Wang N*, Gao J, Liu Y, Shi R, Chen S*. Identification of crucial factors involved in Cynoglossus semilaevis sexual size dimorphism by GWAS and demonstration of zbed1 regulatory network by DAP-seq. Genomics 2022; 114(3): 110376.
5. Zhu Y, Shi R, Yang Q, Zhang M, Chen S, Wang N*. Two nipped-B like protein A (nipbla) gametologs in Chinese tongue sole (Cynoglossus semilaevis): the identification of alternative splicing, expression pattern and promoter activity analysis, Frontiers in Marine Science 2022; 9: 833070.
6. Wang J, Yang Q, Hu Y, Xu W, Yang Y, Chen S*, Wang N*. Identification of lncRNA-miRNA-mRNA network involved in sexual size dimorphism of Chinese tongue sole (Cynoglossus semilaevis). Frontiers in Marine Science 2022; 9: 795525.
7. Yang Q, Li X, Shi R, Cheng P, Wang N*, Chen S*. The female-biased expression, transcriptional regulation and knock-down effect of insulin-like growth factor binding protein 7 in Chinese tongue sole, Cynoglossus semilaevis. Aquaculture 2022; 551: 737956.
8. Wang N*, Yang Q, Wang J, Shi R, Li M, Gao J, Xu W, Yang Y, Chen Y, Chen S*. Integration of transcriptome and methylome highlights the roles of cell cycle and hippo signaling pathway in flatfish sexual size dimorphism. Frontiers in Cell and Developmental Biology 2021; 9:743722.
9. Wang N*, Wang RK, Wang RQ, Chen SL*. Transcriptomics analysis revealing candidate networks and genes for the body size sexual dimorphism of Chinese tongue sole (Cynoglossus semilaevis). Funct Integr Genomics. 2018;18(3): 327-339.
10. Wang R, Zhang N, Wang R, Wang S, Wang N*. Two skin cell lines from wild-type and albino Japanese flounder (Paralichthys olivaceus): establishment, characterization, virus susceptibility, efficient transfection, and application to albinism study. Fish Physiol Biochem. 2017; 43(6):1477-1486.
